Composite

Part:BBa_K1582007

Designed by: Yuwei Zhang   Group: iGEM15_Tianjin   (2015-08-07)
Revision as of 04:09, 8 August 2015 by Registry (Talk | contribs)

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GFP+inJanus-m Fusion Protein

mHGFI is a small protein produced by filamentous fungi, which use serine to replace all of HGFI's conserved eight-cysteine residues.Hydrophobins are amphipathic, which means they have the hydrophobic part and hydrophilic part at the same time. mHGFI is a kind of hydrophobins and it has magic properties. We combine GFP with mHGFI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 910
    Illegal BsaI.rc site found at 644


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Categories
Parameters
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