Composite

Part:BBa_K1582005:Design

Designed by: Chenmao Wang   Group: iGEM15_Tianjin   (2015-08-06)
Revision as of 03:50, 7 August 2015 by Registry (Talk | contribs)

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GFP+sJanus-m Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 976
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI site found at 985


Design Notes

When amplify the HFBI and GFP with PCR, temperature is important.


Source

GFP comes frome iGEM's standard part. We construct the mutant of HFBI ourselves, and the original HFBI comes from Trichoderma reesei.

References