Composite

Part:BBa_S03642:Design

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-12-14)
Revision as of 02:59, 31 January 2007 by Kahaynes (Talk | contribs) (→‎Design Notes)


(-2,-1) HixC-TetB-RBSrev-HixC : pBadrev-HixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1077
    Illegal NheI site found at 1291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 931
    Illegal BamHI site found at 1351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
    Illegal NgoMIV site found at 537
    Illegal NgoMIV site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed RBS-Tet coding region followed by a reversed pBad promoter.

Source

References