RNA

Part:BBa_K1824564

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-06)
Revision as of 12:25, 6 September 2015 by Registry (Talk | contribs)

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RNA Thermometer U6 (Specially designed for T7)

This is a specially designed U6 RNA thermometer that with a unique spacer at the front, which would make it compatible with promoter T7.It is important to noted that this part is not compatible with RFC[10]. XJTLU-CHINA used Gibson method to assembly this part to others.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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