Coding

Part:BBa_K1699003

Designed by: Emil Ruvinov   Group: iGEM15_BGU_Israel   (2015-08-09)
Revision as of 08:27, 3 September 2015 by Shalevgo (Talk | contribs)

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm

Ribozyme flanked gRNA compatible for dCas9-VP64. gRNA sequence complements 3 different loci in the synthetic promoter pMLPm, and gRNA dCas9 complex can promote transcription downstream of syntetic promoter. It has a hammerhead ribozyme on its 5' and an HDV ribozyme on its 3' end. Upon transcription the ribozymes should cleave the mRNA at specific locations to release the mature gRNA.


Usage and Biology

usage and biology


Characterization

characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]



[edit]
Categories
//awards/part_collection/2015
Parameters
None