Composite

Part:BBa_K1824004

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)
Revision as of 17:44, 5 September 2015 by Registry (Talk | contribs)

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pBAD + RNA Thermometer FourU

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer FourU K1824560 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None