Part:BBa_K1720002

Hypoxia-induced promotor

This part is a hypoxia response element.When the cells suffer from hypoxia situation this element will begin to work.It will activate the downstream gene expression.The hypoxia response element is a minimal cis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian cells.

This element with a GFP reporter was then transfected into HEK293 cells .Then we treat the cells with sodium hyposulfite, an reagent that leads to hypoxia. The negative control was HEK293 cells that treat with PBS and we did not detect green fluorescence signal.But we detected green fluorescence signal in the experimental group ,which meant that this element work under hypoxia situation.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 41
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 41
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 41
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 41
  
• 1000
  COMPATIBLE WITH RFC[1000]