Part:BBa_K1824000

Constitutive Promoter J23119 + RNA Thermometer A1

This was a composite regulatory part that consisting of constitutive promoter J23119 and RNA Thermometer A1 for temperature induced gene expression at 42 degrees. A1 was derived from ROSE family (ROSE stands for Repression Of heat Shock gene Expression), first discovered in the 5′ untranslated region of rhizobial heat shock genes (Nocker, 2001). The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination was compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT A1.

References

1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]