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Regulatory
Part:BBa_K1824555
Designed by: Wenbo Xu Group: iGEM15_XJTLU-CHINA (2015-09-04)
Site-directed mutated pLux that permits strong validity
This was a mutated pLux that permitted ideal ON/OFF ratio based on the original promoter Lux pR (BBa_R0062). BBa_R0062 was believed to have high constitutative expression in the absent of 3-oxo-hexanoyl-HSL and this problem went serious when regulatory promoter was coupled with toxins or other repression/activation parts. By using this mutated pLux, genetic circuits would be more easily to control and more sensitive to external signal change.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//promoter
positive_regulators |