Regulatory

Part:BBa_K1824555

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-04)
Revision as of 16:52, 4 September 2015 by Registry (Talk | contribs)

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Site-directed mutated pLux that permits strong validity

This was a mutated pLux that permitted ideal ON/OFF ratio based on the original promoter Lux pR (BBa_R0062). BBa_R0062 was believed to have high constitutative expression in the absent of 3-oxo-hexanoyl-HSL and this problem went serious when regulatory promoter was coupled with toxins or other repression/activation parts. By using this mutated pLux, genetic circuits would be more easily to control and more sensitive to external signal change.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
positive_regulators