Coding

Part:BBa_K1720000:Design

Designed by: YuanbinCui   Group: iGEM15_SCUT-China   (2015-08-09)
Revision as of 08:13, 26 August 2015 by TommyCui (Talk | contribs) (→‎Design Notes)

Human guanylate cyclase1,soluble, alpha 3 unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1498
    Illegal PstI site found at 1827
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1576
    Illegal SapI site found at 1446


Design Notes

This part do not contains promoter so that users can add their own promoter vary from different purposes.

Source

We get the information of Human guanylate cyclase 1,soluble, alpha 3 unit here http://www.ncbi.nlm.nih.gov/gene/2982

References

1. Gheorghiade M, Marti CN, Sabbah HN, Roessig L, Greene SJ, Bohm M, Burnett JC, Campia U, Cleland JG, Collins SP et al: Soluble guanylate cyclase: a potential therapeutic target for heart failure. Heart failure reviews 2013, 18(2):123-134.

2. Sharina IG, Cote GJ, Martin E, Doursout MF, Murad F: RNA splicing in regulation of nitric oxide receptor soluble guanylyl cyclase. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society 2011, 25(3):265-274.

3. Marinko M, Novakovic A, Nenezic D, Stojanovic I, Milojevic P, Jovic M, Ugresic N, Kanjuh V, Yang Q, He GW: Nicorandil directly and cyclic GMP-dependently opens K channels in human bypass grafts. Journal of pharmacological sciences 2015.