Coding

Part:BBa_K1621003:Design

Designed by: Lara Stuehn, Ramona Emig, Julika Neumann, Rabea Jesser   Group: iGEM15_Freiburg   (2015-08-28)
Revision as of 06:29, 30 August 2015 by RamonaE (Talk | contribs) (→‎Source)


TeNT_Hc - C-terminal part of the heavy chain (tetanus toxin)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 430
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E.coli with the codon optimization tool from IDT.


Source

The sequence for this part was obtained from Yu et al. (2010) and synthesized by Integrated DNA Technolgies.

References