Translational_Unit
RBS-xylB
Part:BBa_K1602000
Designed by: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel Group: iGEM15_TU_Darmstadt (2015-08-28)
B0034-xylB
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No part name specified with partinfo tag.
Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation.
To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).
Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
===Usage===
This part is a composite of three genes, each with a strong RBS (BBa_B0034) and all of them under control of one T7 Promoter (BBa_K1497017).
- citrate synthase - gltA (BBa_K1033001)
- aconitate hydratase 1 - acnA (BBa_K1033000)
- cis-aconitate decarboxylase - cadA (BBa_K1497100)
iGEM TU Darmstadt 2014 :)
Figure 2
Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
Sequence and Features
BBa_K1602000 SequenceAndFeatures
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Categories
Parameters
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