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Part:BBa_K1598004:Design

Designed by: Marta Napiorkowska   Group: iGEM15_UCL   (2015-08-27)
Revision as of 21:10, 27 August 2015 by Registry (Talk | contribs)

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RBS-GAD-6xHis-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag - illegal restriction enzyme sites were removed - part was obtained through de novo DNA synthesis



Source

DNA synthesis

References