Regulatory

Part:BBa_K1720002

Designed by: Yuanbin Cui, Yaran Zhao   Group: iGEM15_SCUT-China   (2015-08-25)
Revision as of 08:04, 26 August 2015 by TommyCui (Talk | contribs)

Hypoxia-induced promotor This part is a hypoxia response element. The hypoxia response element is a minimal cis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian cells. This element with a GFP reporter was then transfected into HEK293 cells.We use Vivo green fluorescence signal was observed under fluorescence microscope,

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 41
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 41
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 41
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//regulation
Parameters
biologymammal
switch_pointIt will switch on at hypoxia situation