Coding
Part:BBa_K1763000:Design
Designed by: Daniel Cancilla Group: iGEM15_UCLA (2015-05-21)
Apis mellifera (honeybee) silk fibroin 3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- [http://www.sciencedirect.com/science/article/pii/S0142961209013805 This paper] referred us to the some of the honeybee silk sequences. There are 4 different honeybee silk sequences in native silk that come together to form a silk fiber. However, it was shown that by just expressing one of the three sequences, you could obtain silk with comparable physical properties to the native honeybee silk (Weisman). So we chose Apis mellifera silk fibroin 3 and we synthesized the sequence, making sure to omit the signal peptide at the 5' end of the protein.
Source
- Apis mellifera genome. Reference Genbank FJ235090.1
- Weisman, Sarah, et al. "Honeybee silk: Recombinant protein production, assembly and fiber spinning." Biomaterials 31.9 (2010): 2695-2700.
- Sutherland, Tara D., et al. "Single honeybee silk protein mimics properties of multi-protein silk." PLoS One 6.2 (2011): 16489.
APA