DNA

Part:BBa_K1648000:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-07)
Revision as of 16:11, 14 July 2015 by S1155046866 (Talk | contribs) (→‎Design Notes)


Recombination Template for mamAB Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


Design Notes

In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together.

K1648000 History.png

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References