Composite

Part:BBa_K1638003

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-09)
Revision as of 14:12, 9 May 2015 by Registry (Talk | contribs)

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T25 domain of cyaA from Bordetella pertussis (IPTG inducible)

The T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by IPTG. When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the protein associates, T25 and T18 associates too, and convert ATP to cAMP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 843
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None