Coding

Part:BBa_K1074004

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-12)
Revision as of 12:59, 12 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Protective Antigen Domain 4

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax. Homologues of PA have been found in several spore-forming Gram-positive bacteria, and share the ability to translocate toxic enzymes into the host cytosol.The PA monomer is organised mainly into antiparallel beta-sheets and has four domains: an N-terminal domain (domain 1) containing two calcium ions and the cleavage site for activating proteases; a heptamerisation domain (domain 2) containing a large flexible loop implicated in membrane insertion; a small domain of unknown function (domain 3); and a carboxy-terminal receptor-binding domain (domain 4) [PMID: 9039918].

This entry represents domain 4 of PA, which has an immunoglobulin-like fold. Domain 4 plays a key role in cellular receptor recognition, as well as in pH-dependent pore formation[PMID: 19722284].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 361
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 361
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None