Part:BBa_M36099:Design

E. coli sensor test rig

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2342
  Illegal XbaI site found at 3188
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2342
  Illegal XbaI site found at 3188
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2342
  Illegal XbaI site found at 3188
  Illegal AgeI site found at 2605
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

Design Notes

Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0.

rpo_bla and rrnB1T1T2 transcription terminators serve as insulators.

Source

Gemini coding sequence sourced from Dr. Drew Endy and Martin et al., PlosOne 2009. The rest of the vector is sourced from DNA2.0.

References

PLoS One. 2009 Nov 4;4(11):e7569. doi: 10.1371/journal.pone.0007569. Gemini, a bifunctional enzymatic and fluorescent reporter of gene expression. Martin L, Che A, Endy D.