Coding

Part:BBa_K1499250

Designed by: Raman Nelakanti   Group: iGEM14_StanfordBrownSpelman   (2014-08-27)
Revision as of 20:11, 2 November 2014 by Ashumate (Talk | contribs)

GFP (E0040) with two amber stop codons



Usage and Biology

This part is GFP with two inserted TAG stop codons in place of leucine residues. This part transformed into Amberless cells along with supP tRNA will express GFP. In wild type cells, no GFP will be expressed as the TAG will be recognized as stop codons rather than leucine. This part is used as a proof of concept for Codon Security as part of the Amberless Hell Cell project which mitigates the effects of horizontal gene transfer.

Figure 2. Our approach to the Amberless Hell Cell. By recoding the UAG stop codon to translate into an amino acid, only cells that have a tRNA with the anticodon AUC will produce the complete protein. In our experiment, we used a tRNA that charges with leucine to translate the UAG codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


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Categories
Parameters
None