Part:BBa_K1321334

pSB-AcsAB

This part contains the coding sequence, previously codon-optimised for expression in Escherichia coli, of the Cellulose Synthase enzyme. In Gluconacetobacter xylinus, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such subunits. It is made up of two main domains, a catalytic domain (AcsA) and a regulatory domain (AcsB), which are transcribed and translated in the form of a single, functional unit with both regulatory and catalytic activities. The regulatory domain binds the second messenger c-di-GMP and activates the process, whilst the catalytic domain catalyses the polymerisation of cellulose from UDP-glucose.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1831
  Illegal BglII site found at 2545
  Illegal BglII site found at 4468
  Illegal BamHI site found at 841
  Illegal BamHI site found at 3835
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1817
  Illegal AgeI site found at 1894
  Illegal AgeI site found at 2450
  Illegal AgeI site found at 3251
  Illegal AgeI site found at 3343
  Illegal AgeI site found at 3449
  
• 1000
  COMPATIBLE WITH RFC[1000]