Coding

Part:BBa_K1151001:Experience

Designed by: Davide Magrì   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 05:10, 2 November 2014 by Xen sm (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1151001

User Reviews

UNIQd414e7c9ded928f9-partinfo-00000000-QINU UNIQd414e7c9ded928f9-partinfo-00000001-QINU


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Imperial College 2014

We attempted to use this protein as part of fusions with cellulose binding domains. However after cloning our fusions using this part we noticed that unfortunately this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). To correct the mutation a 'C' needs to be added between bases 52-53. This should be able to be easily achieved using site-directed mutagenesis PCR.

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