Part:BBa_K863101:Experience

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User Reviews

UNIQ8eea1ce11ebbb628-partinfo-00000000-QINU UNIQ8eea1ce11ebbb628-partinfo-00000001-QINU

••••• Imperial College 2014

We successfully used this part: We fused it to sfGFP and expressed the fusion protein and characterised it's binding ability relative to other CBDs as described below: The binding ability of this CBD to bacterial cellulose was characterised when fused to sfGFP, relative to other CBDs fused to sfGFP. The binding ability was represented by the percentage fluorescence remaining from the sfGFP-CBD fusions bound to bacterial cellulose discs, when subjected to various washes (protocol [http://2014.igem.org/Team:Imperial/Protocols here]). When washed with PBS, sfGFP-CBDcex fusions performed the best out of all the CBDs tested (third graph) followed by washes with 5% BSA (fourth graph), dH20 (first graph) and 70% EtOH (second graph). .

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