Translational_Unit

Part:BBa_K1362022

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)
Revision as of 21:50, 17 October 2014 by Cahlmann (Talk | contribs) (Usage and Biology)

RBS + Circular Xylanase

Xylanases are enzymes that degrad Xylan, a major component of wood. This is the circular counterpart of BBa_K1362020. A circular Xylanase should be resistent to exo-peptidases and could be more heat stable because of its reduced conformational space. This could be interesting for industrial applications, as they are often performed at higher temperatures and harsher environments.

Activity after Heatshock assay for 30 minutes of circular Xylanase

Usage and Biology

To characterize our part in respect to its thermal stability we conducted a Heatshock Assay. Firstly the Xylanases were incubated at 37 °C, 50 °C, 56 °C, 60 °C and 66 °C. After 30 minutes substrate (EnzChek® Ultra Xylanase Assay Kit) was added and the fluorescence was measured for 150 minutes in a platereader at 37 °C. The results indicate a reduction of activity at temperatures higher than 50 °C and complete loss of function for temperatures higher than 60 °C.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 557
    Illegal SapI.rc site found at 611


[edit]
Categories
Parameters
None