Part:BBa_J23101

constitutive promoter family member

Variant RFP (au) J23112 1
J23103 17 J23107 908
J23113 21 J23106 1185
J23109 106 J23108 1303
J23117 162 J23118 1429
J23114 256 J23111 1487
J23115 387 J23101 1791
J23116 396 J23104 1831
J23105 623 J23102 2179
J23110 844 J23100 2547

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Constitutive promoter family
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation. See part J61002 for details on their use.

These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for furhter modification. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]