Reporter

Part:BBa_K1555000

Designed by: Xu Xuan   Group: iGEM14_NJAU_China   (2014-09-28)
Revision as of 00:17, 2 November 2014 by Daydayup (Talk | contribs)

File:Https://static.igem.org/mediawiki/parts/1/19/2h 0mg.L copper induce .tif The copa promoter wth a gfp reporter

This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marO（BBa_K1555001） and cueo（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. File:2h 0mg.Lcopper induce.tifFile:Part:BBa K1555000 Kepler186f artistconcept 1.jpg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914


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