Plasmid

Part:BBa_K1391133:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 21:51, 1 November 2014 by Orangethemarker (Talk | contribs)

pENTR_miRNAG1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 502
    Illegal XhoI site found at 398
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 502
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024
    Illegal SapI.rc site found at 16


This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.



Source

Artificial

References