Composite

Part:BBa_K1413024

Designed by: Laura MATABISHI, Nandjafot MENDY   Group: iGEM14_Evry   (2014-10-17)
Revision as of 21:49, 1 November 2014 by Laurabibi (Talk | contribs)

bphR1-RBS-RFP-Terminator
This part is composed by bphR1 promoter, from Pseudomonas pseudoalcaligens KF707, which allows the transcription of the genes of biphenyl degradation, the RBS (BBa_B0034, RFP gene (BBa_E1010) and terminator (BBa_B0015). In our project, genes of degradation was replaced by RFP gene to detect the compound.

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Figure 1: Schema of BBa_K1413024 construction.

Mechanism explanation: In absence of PCBs, bphR2 (BBa_K1413021) is bound to bphR1 promoter which activate the transcription of RFP but in very low expression. In presence of PCBs, when compound diffuses into the media, it binds to bphr2 protein which undergoes a conformational change that permits to activate more stronger bphR1 promoter and increase the transcription of RFP.

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Figure 2 : Mechanism of PCB biosensor with the system bphR2/bphR1 gene

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
    Illegal AgeI site found at 995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


[edit]
Categories
Parameters
None