Composite

Part:BBa_K1413024

Designed by: Laura MATABISHI, Nandjafot MENDY   Group: iGEM14_Evry   (2014-10-17)
Revision as of 20:01, 17 October 2014 by Registry (Talk | contribs)

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bphR1-RBS-RFP-Terminator

bphR1 promoter, from Pseudomonas pseudoalcaligenes KF707, activates the bph genes of biphenyls degradation, in presence of bphR2 protein and biphenyl. In our study, the aim was to detect the presence of polychlorinated biphenyls (PCBs) with RFP fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
    Illegal AgeI site found at 995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


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Categories
Parameters
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