Composite

Part:BBa_K1413023

Designed by: Laura MATABISHI, Nandjafot MENDY   Group: iGEM14_Evry   (2014-10-17)
Revision as of 19:32, 17 October 2014 by Registry (Talk | contribs)

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Promoter-RBS-bphr2 mutated-Terminator

bphR2 gene, from Pseudomonas pseudoalcaligens KF707, allows the transcription of bphR1 which activates the genes of degradation of biphenyls. bphR2 gene has been mutated because of its pstI site in its sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 352


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Parameters
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