Plasmid

Part:BBa_K1391012

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 02:15, 31 October 2014 by Shinjini37 (Talk | contribs)

pENTR_Cofilin (Inactivated - pseudophosphorylated)

Cofilin is an actin depolymerizing factor. It is recruited when PirB/LilrB2 is activated by beta-amyloid. [1]

The 3rd amino acid in this construct has been mutated to make it pseudophophoralated and inactive. It was ordered from Adgene.

[1] Taeho Kim, George S. Vidal, Maya Djurisic, et al. "Human LilrB2 is a beta-amyloid recptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's Model". Science. 20 Sep 2013. <http://www.sciencemag.org/content/341/6152/1399>. 16 Oct 2014.

The characterization of the system this is part of can be found in the experience page.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 268
    Illegal SapI site found at 108


[edit]
Categories
Parameters
None