Part:BBa_K1360001

alpha-L-arabinofuranosidase ArfB

This part is the coding sequence for a thermostable alpha-L-arabinofuranosidase, which specifically releases arabinofuranoside branch of arabinoxylan from xylan.

Usage and Biology

The ArfB enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The optimized working condition is pH = 4.0, temperature = 71℃.

Design considerations

This part is only a coding sequence without expression regulators, and is compatible with RFC10 standards, and the codon is optimized for E.coli expression.

Lab Archives

We use DNS to test the enzymatic activity. DNS is a reagent detecting reductive saccharides. When mannan or xylan is degraded into mannose or xylose, DNS color get dark. When DNS color darker, the enzymatic activity higher.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 679
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1156
  Illegal BsaI.rc site found at 1282
  Illegal BsaI.rc site found at 1372