Part:BBa_K1470002

Galactose-induced gene 4 DNA binding domain (Gal4DBD)

This protein from Saccharomyces cerevisiae is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose [1].
It recognizes a 17 base pair sequence (5'-CGGN₁₁CCG-3') in the upstream activating sequence (UAS-G) of these genes. [2]
GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) [3].
The protein is controlled by a strong promotor. Binding to UAS-G leads to the expression and detection of the corresponding gene, GFP for example.

References

[1] TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496–499.
[2] CAMPBELL, R.N., LEVERENTZ, M.L., RYAN, L.A., REECE, R.J.: Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae. Biochem. J. (2008) 414 (177–187). [3] BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 218
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 137