Plasmid

Part:BBa_K1391114

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-30)
Revision as of 00:48, 31 October 2014 by Registry (Talk | contribs)

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pEXPR hEF1a: LilrB_TCS_Gal4VP16

LilrB2 fused to the recognition sequence (cleavage site) for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator. It is under the hEF1a promoter.

When the TEV cleavage site is cleaved, it releases Gal4-VP16, which can activate the UAS-GAL4 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal XbaI site found at 6043
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
    Illegal NotI site found at 8917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal BglII site found at 4818
    Illegal BglII site found at 8155
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 5424
    Illegal BamHI site found at 5471
    Illegal BamHI site found at 5729
    Illegal BamHI site found at 9112
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 5217
    Illegal XhoI site found at 7790
    Illegal XhoI site found at 8897
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal XbaI site found at 6043
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal XbaI site found at 6043
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal NgoMIV site found at 4952
    Illegal NgoMIV site found at 6334
    Illegal NgoMIV site found at 7027
    Illegal AgeI site found at 3456
    Illegal AgeI site found at 4330
    Illegal AgeI site found at 5866
    Illegal AgeI site found at 6366
    Illegal AgeI site found at 6853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7709
    Illegal BsaI.rc site found at 1121
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 9344
    Illegal SapI.rc site found at 5706


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