Plasmid

Part:BBa_K1391013

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 06:19, 17 October 2014 by Shinjini37 (Talk | contribs)

pENTR_PirB_TCS_Gal4VP16

PirB fused to the recognition sequence (cleavage site) for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator.

When the TEV cleavage site is cleaved, it releases Gal4-VP16, which can activate the UAS-GAL4 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal BamHI site found at 53
    Illegal XhoI site found at 3005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2924
    Illegal SapI.rc site found at 2062


[edit]
Categories
Parameters
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