Measurement

Part:BBa_K1330001

Designed by: Kristian Jensen   Group: iGEM14_DTU-Denmark   (2014-09-30)
Revision as of 20:59, 28 October 2014 by KristianJensen (Talk | contribs)

Spinach2.1 flanked by tRNALys3 with terminator BBa_B0052

Spinach2 mutated with terminator

Usage and Biology

Spinach 2.1 RNA can bind and activate the fluorophore DFHBI, both in vivo and in vitro. This BioBrick also contains a terminator, so it can be placed after a promoter to measure its activity. The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal. It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the Anderson promoter library. This part does not contain or need a Ribosome Binding Site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None