Generator
WOG

Part:BBa_J45100:Design

Designed by: Boyuan Zhu   Group: iGEM06_MIT   (2006-08-09)
Revision as of 04:25, 31 October 2006 by Drephd (Talk | contribs) (→‎Design Notes)

Wintergreen odor enzyme (BSMT1) generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287


Design Notes

BSMT CDS taken from GenBank. The following primers were used for cloning:

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'

  • LENGTH: 19
  • GC CONTENT: 36.8 %
  • MELT TEMP: 47.8 ºC


Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

  • LENGTH: 22
  • GC CONTENT: 27.3 %
  • MELT TEMP: 46.6 ºC


Source

Species: Petunia X. hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>