Plasmid

Part:BBa_K1391112:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 03:02, 18 October 2014 by Registry (Talk | contribs)

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pEXPR_Tret:Cofilin (Inactivated - pseudophosphorylated)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 954
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
    Illegal NotI site found at 926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 954
    Illegal BglII site found at 12
    Illegal XhoI site found at 921
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
    Illegal NgoMIV site found at 1907
    Illegal NgoMIV site found at 3248
    Illegal NgoMIV site found at 3531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 1233
    Illegal BsaI.rc site found at 5056
    Illegal SapI site found at 1073
    Illegal SapI site found at 3976
    Illegal SapI.rc site found at 3097
    Illegal SapI.rc site found at 3307


Design Notes

None


Source

Human

References