Conjugation
Part:BBa_K1510922
Designed by: Kao Jung Chang Group: iGEM14_NYMU-Taipei (2014-10-09)
Fragrance generator part1: ketoisocaproate decarboxylase (THI3) and RBS
encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction.BBa_B0034(RBS)can not only facilitate the transcription rate but also bind to the other two fragrance generators.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 21
Illegal EcoRI site found at 922
Illegal EcoRI site found at 1621
Illegal EcoRI site found at 1807 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 21
Illegal EcoRI site found at 922
Illegal EcoRI site found at 1621
Illegal EcoRI site found at 1807 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 21
Illegal EcoRI site found at 922
Illegal EcoRI site found at 1621
Illegal EcoRI site found at 1807 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 21
Illegal EcoRI site found at 922
Illegal EcoRI site found at 1621
Illegal EcoRI site found at 1807 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 21
Illegal EcoRI site found at 922
Illegal EcoRI site found at 1621
Illegal EcoRI site found at 1807 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |