Conjugation

Part:BBa_K1510119

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-07)
Revision as of 18:20, 8 October 2014 by CKJsplendid (Talk | contribs)

A truncated INPNC anchor protein that binds fragments on the cell surface

INPNC is an anchor protein that binds non-specific product on surface.

we use KOD PCR to trunk part k523013(designed by Edinburgh 2011 igem team) to get Plac+Lacz constitutive promoter,RBS and surface display protein INPNC DNA sequence. The linking site at the end of INPNC is free to attach any moderate desired product with designed primer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None