Regulatory

Part:BBa_K1440066

Designed by: Xuanye Cao   Group: iGEM14_Fudan   (2014-10-07)
Revision as of 03:04, 24 October 2014 by Eric423627 (Talk | contribs)

reverse CMV->reverse RFP->pTRE->shRNA


this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1184
    Illegal AgeI site found at 13
    Illegal AgeI site found at 125
    Illegal AgeI site found at 1116
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None