Generator

Part:BBa_K1499004

Designed by: Raman Nelakanti   Group: iGEM14_StanfordBrownSpelman   (2014-10-08)
Revision as of 18:47, 24 October 2014 by Rdispenza (Talk | contribs)

Cellulose binding domains with streptavidin domain generator

This part encodes a protein with two cellulose-binding domains on either end joined by a streptavidin domain.


Usage and Biology

The idea behind this part is that each domain binds to a single cellulose fiber, thus providing a way to cross-link and strengthen cellulose polymers. In addition, the streptavidin domain allows for the modular addition of biotinylated proteins through protein-protein interactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 703
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 304
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 651

Characterization

Verification of Part

The part was sequence verified before submission to the registry with two reads using VF2 and VR (Figure 1).

(figure here)


[edit]
Categories
//binding/cellulose
Parameters
None