Part:BBa_K1470006

pMIG

Template pMIG

Usage and Biology

pMIG is a novel and easy to handle mammalian expression vector and to create fast and efficiently stable cell lines. It contains Long Terminal Repeats (LTR) for production of viral vectors. There is no packaging signal or gag, pol, env in pMIG. Thus it can be used in any S1 laboratory.

Because of pMIGs retroviral origins, it can be used only in packaging cell lines such as Phoenix cells, to produce viral vectors. Otherwise there won’t be any functional vectors due to the lack of gag, pol, env and the psi-packaging signal.

Sequence and Features

There is a multiple cloning site between the LTRs including EcoRI,PstI,BamHI and many more so you can insert any gene of interest. We suggest easy and reasonable digestion and ligation of an EcoRI- and PstI-site free plasmid backbone.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4917
  Illegal XbaI site found at 289
  Illegal XbaI site found at 3711
  Illegal PstI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Unknown
• 21
  INCOMPATIBLE WITH RFC[21]

  Unknown
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4917
  Illegal XbaI site found at 289
  Illegal XbaI site found at 3711
  Illegal PstI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4917
  Illegal XbaI site found at 289
  Illegal XbaI site found at 3711
  Illegal PstI site found at 10
  Illegal NgoMIV site found at 3466
  Illegal NgoMIV site found at 4123
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 520
  Illegal BsaI site found at 541
  Illegal BsaI site found at 3964
  Illegal BsaI.rc site found at 1901
  Illegal BsaI.rc site found at 4030
  Illegal BsaI.rc site found at 4645
  Illegal SapI site found at 818