Translational_Unit

Part:BBa_K1321335:Design

Designed by: Laura de Arroyo Garcia   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 16:22, 21 October 2014 by Geoben (Talk | contribs)

acsCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1837
    Illegal BglII site found at 4382
    Illegal BamHI site found at 1906
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 778
    Illegal AgeI site found at 1334
    Illegal AgeI site found at 1913
    Illegal AgeI site found at 2123
    Illegal AgeI site found at 2975
    Illegal AgeI site found at 3293
    Illegal AgeI site found at 3638
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both genes have the B0034 RBS


Source

Protein coding sequence from genome of Gluconacetobacter Xylinus, resynthesized and codon optimised for E. coli

References