Part:BBa_K1323014

Xylose Inducible Promoter for Staphylococcus aureus

This sequence contains the T5X xylose inducible promoter and the xylose promoter repressor, XylR, for Staphylococcus. It has been shown by Forsyth et al. (2002) that 2% xylose in LB is sufficient to generate significant expression downstream of this promoter (Forsyth et al, 2002). In the absence of xylose, XylR represses activity by binding to the promoter region. Furthermore, glucose was shown to repress the activity of this promoter (Wieland et al., 1995). Our team obtained the sequence for this part from Dr. Donegan from the Ambrose Lab at The Geisel School of Medicine, Dartmouth. This part was synthesized from Bio Basic. By doing so, we were able to remove 3 illegal sites.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1476
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Forsyth RA, et al., 2002. A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol 43:1387–1400.

Wieland, K.P, Wieland, B. Gotz, F. 1995. A promoter screening plasmid and xylose inducible glucose-repressible expression vectors for Staphylococcus carnosus. Gene. 158: 91- 96.