Coding

Part:BBa_K1321107:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 20:00, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)


CBDcen-linker fused to Phytochelatin (PC) EC20 with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CBD15+PhytoC


Source

Phytochelatin and CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References