Composite

Part:BBa_K1336007

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-10)
Revision as of 11:44, 10 October 2014 by Registry (Talk | contribs)

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LacI Expression Cassette with BsDyP gene

BsDyP gene (1251bp) from Bacillus subtilis that has been found to be effective in degrading lignin and azo dyes, in an oxidative process. This gene is ligated to a LacI expression cassette, IPTG inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1741
    Illegal BsaI site found at 1750
    Illegal BsaI site found at 2716
    Illegal SapI site found at 2146
    Illegal SapI site found at 2504
    Illegal SapI.rc site found at 2660


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Parameters
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