Other
Part:BBa_K1336005:Design
Designed by: Yan-Kay Ho Group: iGEM14_UCL (2014-10-06)
Antisense for octaprenyl diphosphate synthase (ispB gene)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When designing PCR primers to change the reverse complement sequence of the ispB gene into the BioBrick format, we truncated the fragment to avoid an illegal PstI restriction site (and so no extra primers were required for site-directed mutagenesis to remove illegal sites).
Source
From genomic sequence of Escherichia coli.