Part:BBa_K1371015
pT7+birA+accA+pccB+2TM-pT7+sfp+2TM
Substrate supply
There are three enzymes needed in our chassis. Propionyl-CoA carboxylase(pcc), an enzyme needed in the biosynthesis of polyketides, is an enzyme complex containing two components playing the core role in our chassis, of which accA is the α biotinylated subunit functioning as the acetyl-CoA or propionyl-CoA carboxylase while pccB,the β subunit,acts as the carboxyl transferase[1].
BirA
BirA is a biotin ligase that improves the activity of accA, acts as an enhancer like sfp, the phosphopantetheinyl transferase facilitating posttranslational modification of the DEBS proteins in E.coli BL21(DE3) [2]. With the pcc and sfp genes transformed into E.coli BL21(DE3), it will express DEBS genes at the comparable level to S. erythraea or S.coelicolor [2].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2946
Illegal BglII site found at 3362
Illegal BamHI site found at 1489 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1880
Illegal NgoMIV site found at 1895
Illegal NgoMIV site found at 3610
Illegal NgoMIV site found at 3687
Illegal AgeI site found at 2252
Illegal AgeI site found at 2384
Illegal AgeI site found at 2579 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1712
Illegal BsaI.rc site found at 2426
Illegal BsaI.rc site found at 3826
Illegal SapI.rc site found at 2039
n/a | pT7+birA+accA+pccB+2TM-pT7+sfp+2TM |