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Part:BBa_K1456002:Experience

Designed by: Mustafa Semih Elitok   Group: iGEM14_ATOMS-Turkiye   (2014-07-21)
Revision as of 18:31, 20 October 2014 by Mustafa26 (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K1456002

ATOMS TURKİYE 2014

                        ATOMS_gpx_results_16.jpg

The amounts of protein acquired were as the charts above and the these data were taken into consideration in the preparation of the assays. 

                        ATOMS_gpx_results_17.jpg

A functional assay of GPx was done through NADPH to measure GPx activity. The same amount of genes were transferred to all samples but varying amount of Na2SeO3 was prepared in the samples, with 2 samples containing different Na2SeO3 concentrations and one control group. The GPx gene was not transfected to the control group. From these 3 groups, the assay of the control group gave the lowest yield as expected; the sample of 500 nM Na2SeO3 produced the mid-result. The sample of 1000 nM Na2SeO3 yielded the highest activity results in the assay. These results prove that the GPx production is directly proportional to the amount of added Na2Se2O3 and that the GPx enzyme was produced functionally.


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